Publications

Side Chain Dynamics of the Trifluoroacetone Cysteine Derivative Characterized by ¹⁹F NMR Relaxation and Molecular Dynamics Simulations
Suad Rashid, Brian L. Lee, Benjamin Wajda, and Leo Spyracopoulos.
Journal of Physical Chemistry B, 123 (2019) 3665-3671. doi:10.1021/acs.jpcb.9b01741

The Hsp90 Chaperone: ¹H and ¹⁹F Dynamic Nuclear Magnetic Resonance Spectroscopy Reveals a Perfect Enzyme
Brian L. Lee, Suad Rashid, Annemarie Wolmarans, Paul LaPointe, and Leo Spyracopoulos.
Biochemistry, 58 (2019) 1869-1877. doi:10.1021/acs.biochem.9b00144

Role of Polyubiquitin Chain Flexibility in the Catalytic Mechanism of Cullin-RING Ligases
Craig J. Markin, Pascal Mericer, and Leo Spyracopoulos.
Journal of Physical Chemistry B, 123 (2019) 776-786. doi:10.1021/acs.jpcb.8b10706

Dynamics-Derived Insights into Complex Formation between the CXCL8 Monomer and CXCR1 N-Terminal Domain: An NMR Study
Prem Raj B. Joseph, Leo Spyracopoulos, and Krishna Rajarathnam.
Molecules, 23: 2825 (2018) 1-20. doi:10.3390/molecules23112825

Active Site Gate Dynamics Modulate the Catalytic Activity of the Ubiquitination Enzyme E2-25K
Manoj K. Rout, Brian L. Lee, Aiyang Lin, Wei Xiao, and Leo Spyracopoulos.
Scientific Reports, 8:7002 (2018) 1-15. doi:10.1038/s41598-018-25476-8, Full text access

RYBP Is a K63-Ubiquitin-Chain-Binding Protein that Inhibits Homologous Recombination Repair.
Mohammad A.M. Ali, Hilmar Strickfaden, Brian L. Lee, Leo Spyracopoulos, and Michael Hendzel.
Cell Reports, 22 (2018) 383-395. doi:10.1016/j.celrep.2017.12.047

Molecular Basis for K63-linked Ubiquitination Processes in Double-Strand DNA Break Repair: A Focus on Kinetics and Dynamics.
Brian Lee, Anamika, J.N. Mark Glover, Michael Hendzel, and Leo Spyracopoulos.
Journal of Molecular Biology, 429 (2017) 3409-3429. doi:10.1016/j.jmb.2017.05.029

The Mechanism of Hsp90 ATPase Stimulation by Aha1.
Anne Marie Wolmarans, Brian Lee, Leo Spyracopoulos, and Paul LaPointe.
Scientific Reports, 6 doi:10.1038/srep33179 (2016)

Ubc13 - The Lys63 Ubiquitin Chain Building Machine.
Curtis D. Hodge, Leo Spyracopoulos, and J.N. Mark Glover.
Oncotarget, 10.18632/oncotarget.10948 (2016)

The Proteasome: More Than a Means to an End.
Leo Spyracopoulos.
Structure, 24 (2016) 1221-1223

Molecular Basis for Phosphorylation Dependent SUMO Recognition by the DNA Repair Protein RAP80.
Anamika and Leo Spyracopoulos.
The Journal of Biologcial Chemistry, 291 (2016) 4417-4428

Covalent Inhibition of Ubc13 Affects Ubiquitin Signaling and Reveals Active Site Elements Important for Targeting.
Curtis D. Hodge, Ross Edwards, Craig J. Markin, Darrin McDonald, Mary Pulvino, Michael Huen, Jiyong Zhao, Leo Spyracopoulos, Michael Hendzel, and J.N. Mark Glover.
ACS Chemical Biology, 10 (2015) 1718-1728

Stochastic Gate Dynamics Regulate the Catalytic Activity of Ubiquitination Enzymes.
Manoj K. Rout, Curtis D. Hodge, Craig J. Markin, Xin Xu, J.N. Mark Glover, Wei Xiao, and Leo Spyracopoulos.
Journal of the American Chemical Society, 136 (2014) 17446-17458. DOI: 10.1021/ja505440b

A Mutation in the Catalytic Loop of Hsp90 Specifically Impairs ATPase Stimulation by Aha1p but not Hch1p.
Natalie K. Horvat, Heather Armstrong, Brian L. Lee, Rebecca Mercier, Annemarie Wolmarans, Jacob Knowles, Leo Spyracopoulos, and Paul LaPointe.
Journal of Molecular Biology, 426 (2014) 2379-2392

Molecular Basis for Impaired DNA Damage Response Function Associated with the RAP80 ΔE81 Defect.
Anamika, Craig J. Markin, Manoj K. Rout and Leo Spyracopoulos.
The Journal of Biological Chemistry, 289 (2014) 12852-12862

Structural Basis of Prion Inhibition by Phenothiazine Compounds.
Pravas Kumar Baral, Mridula Swayampakula, Manoj Kumar Rout, Nat N.V. Kav, Leo Spyracopoulos, Adriano Aguzzi, and Michael N.G. James.
Structure, 22 (2014) 291-303

Accuracy and Precision of Protein-Ligand Interaction Kinetics Determined from Chemical Shift Titrations.
Craig J. Markin and Leo Spyracopoulos.
Journal of Biomolecular NMR, 54 (2012) 355-376

Inreased Precision for Analysis of Protein-Ligand Dissociation Constants Determined from Chemical Shift Titrations.
Craig J. Markin and Leo Spyracopoulos.
Journal of Biomolecular NMR, 53 (2012) 125-138

UBE4B Promotes Hdm2-Mediated Degradation of the Tumor Suppressor p53.
Hong Wu, Scott L. Pomeroy, Manuel Ferreira, Natalia Teider, Juliana Mariani, Keicchi I. Nakayama, Shigetsugu Hatakeyama, Victor A. Tron, Linda F. Saltibus, Leo Spyracopoulos, and Roger P. Leng.
Nature Medicine, 17 (2011) 347-355, doi:10.138/nm.2283

Catalytic Proficiency of Ubiquitin Conjugation Enzymes: Balancing pKa Suppression, Entropy, and Electrostatics.
Craig J. Markin, Linda F. Saltibus, Melissa J. Kean, Ryan T. McKay, Wei Xiao, and Leo Spyracopoulos.
Journal of the American Chemical Society, 132 (2010) 17775-17786

Context-dependent remodeling of structure in two large protein fragments.
Matthew J. Schellenberg, Dustin B. Ritchie, Tao Wu, Craig J. Markin, Leo Spyracopoulos, and Andrew M. MacMillan.
Journal of Molecular Biology, 402 (2010) 720-730

Mechanism for recognition of polyubiquitin chains: Balancing affinity through interplay between multivalent binding and dynamics.
Craig J. Markin, Wei Xiao, and Leo Spyracopoulos.
Journal of the American Chemical Society, 132 (2010) 11247-11258

Dynamics of the RING Domain from Human TRAF6 by ¹⁵N NMR Spectroscopy: Implications for Biological Function.
Craig J. Markin, Linda F. Saltibus, and Leo Spyracopoulos.
Biochemistry, 47 (2008) 10010-10017

NMR Studies of the Dynamics of a Bifunctional Rhodamine Probe Attached to Troponin C.
Oliver Julien, Pascal Mercier, Leo Spyracopoulos, John Corrie, and Brian D. Sykes.
Journal of the American Chemical Society, 130 (2008) 2602-2609

Two Mms2 Residues Cooperatively Interact with Ubiquitin and are Critical for the Lys63 Polyubiquitination in vitro and in vivo.
Landon Pastushok, Leo Spyracopoulos, and Wei Xiao.
FEBS Letters, 581 (2007) 5343-5348

Structure, Interactions, and Dynamics of the RING Domain from Human TRAF6.
Pascal Mercier, Michael J. Lewis, D. Duong Hau, Linda F. Saltibus, Wei Xiao, and Leo Spyracopoulos.
Protein Science, 16 (2007) 602-614

Probing Nascent Structures in Peptides Using Natural Abundance ¹³C NMR Relaxation and Reduced Spectral Density Mapping.
Carolyn M. Slupsky, Leo Spyracopoulos, Valerie K. Booth, Brian D. Sykes, and Matthew P. Crump.
Proteins: Structure, Function, and Bioinformatics, 67 (2007) 18-30

A Suite of Mathematica Notebooks for the Analysis of Protein Main Chain ¹⁵N NMR Relaxation Data.
Leo Spyracopoulos.
Journal of Biomolecular NMR, 36 (2006) 215-224

Structure and Interactions of the Ubiquitin-Conjugating Enzyme Variant Human Uev1a: Implications for Enzymatic Synthesis of Polyubiquitin Chains.
D. Duong Hau, Michael J. Lewis, Linda F. Saltibus, Landon Pastushok, Wei Xiao, and Leo Spyracopoulos.
Biochemistry, 45 (2006) 9866-9877. PDF

Structural Basis for Non-Covalent Interaction between Ubiquitin and the Ubiquitin Conjugating Enzyme Variant Human Mms2.
Michael J. Lewis, Linda F. Saltibus, D. Duong Hau, Wei Xiao, and Leo Spyracopoulos.
Journal of Biomolecular NMR, 34 (2006) 89-100

Main Chain and Side Chain Dynamics of the Ubiquitin Conjugating Enzyme Variant Human Mms2 in the Free and Ubiquitin-bound States.
Leo Spyracopoulos, Michael J. Lewis, and Linda F. Saltibus.
Biochemistry, 44 (2005) 8770-8781. PDF

Thermodynamic Interpretation of Protein Dynamics from NMR Relaxation Measurements.
Leo Spyracopoulos.
Protein and Peptide Letters, 12 (2005) 235-240

The Structure of an Allosteric Inhibitor of LFA-1 Bound to the I-domain Studied by Crystallography, NMR, and Calorimetry.
Matthew P. Crump, Thomas A. Ceska, Leo Spyracopoulos, Alistair Henry, Sarah C. Archibald, Rikki Alexander, Richard J. Taylor, Stuart C. Findlow, James O'Connell, Martyn K. Robinson, and Anthony Shock.
Biochemistry, 43 (2004) 2394-2404

Interaction of a Peptide from the Receptor-Binding Domain of Pseudomonas aeruginosa Pili Strain PAK with a Cross-Reactive Antibody: Changes in Backbone Dynamics Induced by Binding.
A. Patricia Campbell, Leo Spyracopoulos, Wah Y. Wong, Randall T. Irvin, and Brian D. Sykes.
Biochemistry, 42 (2003) 11334-11346

Energetics and Specificity of Interactions within Ub-UEV-Ubc13 Human Ubiquitin Conjugation Complexes.
Sean McKenna, Jing Hu, Trevor Moraes, Wei Xiao, Michael J. Ellison, and Leo Spyracopoulos.
Biochemistry, 42 (2003) 7922-7930. PDF

An NMR Based Model of the Ubiquitin-Bound Human Ubiquitin Conjugation Complex Mms2/Ubc13: The Structural Basis for Lysine-63 Chain Catalysis.
Sean McKenna, Trevor Moraes, Landon Pastushok, Wei Xiao, Christopher Ptak, Leo Spyracopoulos, and Michael J. Ellison.
Journal of Biological Chemistry, 278 (2003) 13151-13158

Spruce Budworm Antifreeze Protein: Changes in Structure and Dynamics at Low Temperature.
Steffen P. Graether, Stéphane M. Gagné, Leo Spyracopoulos, Zongchao Jia, Peter L. Davies, and Brian D. Sykes.
Journal of Molecular Biology, 327 (2003) 1155-1168

Structure and Dynamics of a Beta-helical Antifreeze Protein.
Margaret E. Daley, Leo Spyracopoulos, Zongchao Jia, Peter L. Davies, and Brian D. Sykes.
Biochemistry, 41 (2002) 5515-5525

Non-covalent Interaction Between Ubiquitin and the DNA Repair Protein Mms2 is Required for Ubc13-mediated Poly-ubiquitination.
Sean McKenna, Leo Spyracopoulos, Trevor Moraes, Landon Pastushok, Christopher Ptak, Wei Xiao, and Michael J. Ellison.
Journal of Biological Chemistry, 276 (2001) 40120-40126

Thermodynamic Insights into Proteins from NMR Spin Relaxation Studies.
Leo Spyracopoulos and Brian D. Sykes.
Current Opinion in Structural Biology, 11 (2001) 555-559

Temperature Dependence of Dynamics and Thermodynamics of the Regulatory Domain of Human Cardiac Troponin C.
Leo Spyracopoulos, Pierre Lavigne, Matthew P. Crump, Stéphane M. Gagné, Cyril M. Kay, and Brian D. Sykes.
Biochemistry, 40 (2001) 12541-12551. PDF

Structure, Dynamics, and Thermodynamics of the Structural Domain of Troponin C in Complex with the Regulatory Peptide 1-40 of Troponin I.
Pascal Mercier, Leo Spyracopoulos, and Brian D. Sykes.
Biochemistry, 40 (2001) 10063-10077

Structure of the C-domain of Human Cardiac Troponin C in Complex with the Ca²⁺ Sensitizing Drug EMD57033.
Xu Wang, Monica X. Li, Leo Spyracopoulos, Norbert Beier, Murali Chandra, R. John Solaro, and Brian D. Sykes.
Journal of Biological Chemistry, 276 (2001) 25456-25466

Backbone Dynamics of Receptor Binding and Antigenic Regions of a Pseudomonas Aeruginosa Pilin Monomer.
J.-Y. Suh, Leo Spyracopoulos, David W. Keizer, Randall T. Irvin, and Brian D. Sykes.
Biochemistry, 40 (2001) 3985-3995

Complete ¹H, ¹³C, and ¹⁵N Backbone Assignments for the Hepatitis A Virus 3C Protease.
Trent Bjorndahl, M. Scott Watson, Carolyn M. Slupsky, Leo Spyracopoulos, Brian D. Sykes, and David S. Wishart.
Journal of Biomolecular NMR, 19 (2001) 187-188

NMR Analysis of Type III Antifreeze Protein Intramolecular Dimer: Structural Basis for Enhanced Activity.
Kazunori Miura, Satoru Ohgiya, Tamotsu Hoshino, Nobuaki Nemoto, Tetsuya Suetake, Ai Miura, Leo Spyracopoulos, Hidemasa Kondo, and Sakae Tsuda.
Journal of Biological Chemistry, 276 (2001) 1304-1310

Backbone Dynamics of a Bacterially Expressed Peptide from the Receptor Binding Domain of Pseudomonas Aeruginosa Pili Strain PAK from heteronuclear ¹H-¹⁵N NMR Spectroscopy
A. Patricia Campbell, Leo Spyracopoulos, Randall T. Irvin, and Brian D. Sykes.
Journal of Biomolecular NMR, 7 (2000) 239-255

Interaction of Cardiac Troponin-C with Ca²⁺ Sensitizor EMD57033 and cardiac Troponin-I Inhibitory Peptide.
Monica X. Li, Leo Spyracopoulos, Norbert Beier, John A. Putkey, and Brian D. Sykes.
Biochemistry, 39 (2000) 8782-8790

Backbone Dynamics of the Human CC Chemokine Eotaxin: Fast Motions, Slow Motions and Implications for Receptor Binding.
Matthew P. Crump, Leo Spyracopoulos, Pierre Lavigne, Key-Sun Kim, Ian Clark-Lewis, and Brian D. Sykes.
Protein Science, 8 (1999) 2041-2054

Know Your Protein: Effects of Weak Dimerization.
Leo Spyracopoulos, Stéphane M. Gagné, and Brian D. Sykes.
Proceedings for The International School of Structural Biology and Magnetic Resonance, 4^th Course on Dynamics, Structure, and Function of Biological Macromolecules. eds. O. Jardetzky and J.F. Lefèvre, Plenum Press, New York, NY, USA (1999)

Binding of Cardiac Troponin-I_147-163 Induces a Structural Opening in Human Cardiac Troponin-C.
Monica X. Li, Leo Spyracopoulos, and Brian D. Sykes.
Biochemistry, 38 (1999) 8289-8298

Low Temperature-Induced Structural Changes in the Apo Regulatory Domain of Skeletal Muscle Troponin C.
Sakae Tsuda, Ai Miura, Stéphane M. Gagné, Leo Spyracopoulos, and Brian D. Sykes.
Biochemistry, 38 (1999) 5693-5700

Dynamics and Thermodynamics of the Regulatory Domain of Human Cardiac Troponin C in the Apo- and Calcium- Saturated States.
Leo Spyracopoulos, Stéphane M. Gagné, Monica X. Li, and Brian D. Sykes.
Biochemistry, 37 (1998) 18032-18044

Backbone and Methyl Dynamics of the Regulatory Domain of Troponin C: Anisotropic Rotational Diffusion and Contribution of Conformational Entropy to Calcium Affinity.
Stéphane M. Gagné,Sakae Tsuda, Leo Spyracopoulos, Lewis E. Kay and Brian D. Sykes.
Journal of Molecular Biology, 278 (1998) 667-686

NMR Studies of Protein Sidechain Dynamics: Examples from Antifreeze and Calcium-Regulatory Proteins.
Leo Spyracopoulos, Stéphane M. Gagné, Wolfram Gronwald, Lewis E. Kay, and Brian D. Sykes.
Proceedings for The International School of Structural Biology and Magnetic Resonance, 3^rd Course on Protein Dynamics, Function and Design. eds. O. Jardetzky and J.F. Lefèvre, Plenum Press, New York, NY, USA (1998) 147-162

NMR Studies of Ca²⁺ Binding to the Regulatory Domains of Cardiac and E41A Skeletal Muscle Troponin C Reveal the Importance of Site I to Energetics of the Induced Structural Changes.
Monica X. Li, Stéphane M. Gagné, Leo Spyracopoulos, Cathelijne P.A.M. Kloks, Gerald Audette, Murali Chandra, R. John Solaro, Lawrence B. Smillie, and Brian D. Sykes.
Biochemistry, 36 (1997) 12512-12525

Calcium-Induced Structural Transition in the Regulatory Domain of Human Cardiac Troponin C
Leo Spyracopoulos, Monica X. Li, Samuel K. Sia, Stéphane M. Gagné, Murali Chandra, R. John Solaro, and Brian D. Sykes.
Biochemistry, 36 (1997) 12138-12146

Structure of Cardiac Muscle Troponin C Unexpectedly Reveals a Closed Regulatory Domain
Samuel K. Sia, Monica X. Li, Leo Spyracopoulos, Stéphane M. Gagné, Wen Liu, John A. Putkey, and Brian D. Sykes.
The Journal of Biological Chemistry, 272 (1997) 18216-18221

Backbone Dynamics of an Alamethicin in Methanol and Aqueous Detergent Solution Determined by Heteronuclear ¹H-¹⁵N NMR Spectroscopy
Leo Spyracopoulos, Adelinda Yee, and Joe D. J. O'Neil
Journal of Biomolecular NMR, 7 (1996) 283-294

Effect of a Hydrophobic Environment on the Hydrogen Exchange Kinetics of Model Amides Determined by ¹H-NMR Spectroscopy
Leo Spyracopoulos and Joe D. J. O'Neil
Journal of the American Chemical Society, 116 (1994) 1395-1402